Ha Van Huan, Yong Wang, Siliang Zhang (2012). Down-Regulation of Pm4CL1 Gene Expression in E. coli Model by Antisense mRNA. Biochemical Genetics (Springer publisher) 50(7-8):529-537


Được đăng: Ngày 21 Tháng 10 Năm 2015 10:10 | Viết bởi Hà Văn Huân (Chủ nhiệm Dự án) |

Abstract: The coumarate:coenzyme A ligase 1 gene (4CL1) was isolated from sec- ondary developing xylem tissues of Pinus massoniana Lamb (Pm4CL1). The Pm4CL1 gene was inserted into a pQE30 expression vector in both sense and antisense orienta- tions to form a pQE30-4CL1-anti4CL1. The expression level of the Pm4CL1 gene in Escherichia coli cells was inhibited by the antisense mRNA of the Pm4CL1 gene. The results of reverse transcriptase polymerase chain reaction and sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the average mRNA and protein expression levels of the Pm4CL1 gene in E. coli cells were decreased by 43 and 46%, respectively. These results are an important basis for further investigation

Introduction